Evol Ecol Res 15: 295-311 (2013)     Full PDF if your library subscribes.

Are Japanese freshwater populations of threespine stickleback derived from the Pacific Ocean lineage?

Lara M. Cassidy1,2, Mark Ravinet1,3, Seiichi Mori4 and Jun Kitano1,5

1Ecological Genetics Laboratory, National Institute of Genetics, Mishima, Japan,  2Trinity College, Dublin, Ireland,  3Queen’s University Belfast, Belfast, UK,  4Gifu-keizai University, Ogaki, Japan and  5PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan

Correspondence: J. Kitano, Ecological Genetics Laboratory, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411–8540, Japan.
e-mail: jkitano@lab.nig.ac.jp


Background: The presence of ecological opportunity can trigger adaptive radiation. Freshwater colonization of marine ancestors during the post-glacial dispersal could have triggered adaptive radiation in the threespine stickleback (Gasterosteus aculeatus). Japanese marine threespine stickleback can be classified into two genetically divergent groups, the Pacific Ocean group and the Japan Sea group.

Question: Are all Japanese freshwater threespine stickleback populations derived from the Pacific Ocean lineage? Or are some freshwater populations derived from the evolutionarily divergent Japan Sea lineage?

Methods: We collected stickleback from 22 different locations across Japan, including nine freshwater populations, five Pacific Ocean anadromous populations, and eight Japan Sea anadromous populations. We determined the genotypes for 11 different microsatellite markers. We created phylogenies using different measures of genetic distance based on both allele frequencies and allele lengths. We inferred population structure using Bayesian analysis.

Results: All freshwater populations analysed were genetically similar to the Pacific Ocean anadromous populations, suggesting that they are likely derived from the Pacific Ocean lineage rather than the Japan Sea lineage.

Keywords: adaptive radiation, microsatellites, phylogeography, stickleback.

IF you are connected using the IP of a subscribing institution (library, laboratory, etc.)
or through its VPN.


        © 2013 Jun Kitano. All EER articles are copyrighted by their authors. All authors endorse, permit and license Evolutionary Ecology Ltd. to grant its subscribing institutions/libraries the copying privileges specified below without additional consideration or payment to them or to Evolutionary Ecology, Ltd. These endorsements, in writing, are on file in the office of Evolutionary Ecology, Ltd. Consult authors for permission to use any portion of their work in derivative works, compilations or to distribute their work in any commercial manner.

       Subscribing institutions/libraries may grant individuals the privilege of making a single copy of an EER article for non-commercial educational or non-commercial research purposes. Subscribing institutions/libraries may also use articles for non-commercial educational purposes by making any number of copies for course packs or course reserve collections. Subscribing institutions/libraries may also loan single copies of articles to non-commercial libraries for educational purposes.

       All copies of abstracts and articles must preserve their copyright notice without modification.