Evol Ecol Res 9: 775-787 (2007)     Full PDF if your library subscribes.

Seasonal resource oscillations maintain diversity in bacterial microcosms

Christine C. Spencer,1* Gerda Saxer,3 Michael Travisano3 and Michael Doebeli1,2

1Department of Zoology and  2Department of Mathematics, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada and  3Department of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA

Address all correspondence to Christine C. Spencer, Department of Ecology and Evolutionary Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario M5S 3G5, Canada.
e-mail: cspencer@eeb.utoronto.ca


Hypothesis: Seasonal variation in availability of resources maintains co-existence between different ecological types due to frequency-dependent interactions.

Organism: Escherichia coli.

Methods: When populations containing two ecotypes of E. coli that evolved from a common ancestor under sympatric conditions grow in batch culture on a mixture of glucose and acetate, they first use up the available glucose and then switch to acetate consumption, thus creating a fluctuating environment with two different ‘seasons’. We removed the alternation between the seasons so that polymorphic populations only experienced the first (glucose) season or the second (acetate) season and monitored the frequency of the ecotypes. We subsequently removed the stationary phase of the batch culture to determine its contribution to competitive interactions between the two ecotypes. (During the stationary phase, a population stops growing because it has depleted its resources and produced too high a level of toxins.)

Results: We show that for two stable, heritable ecotypes of the bacterium E. coli co-existing on two seasonally available resources, the removal of either resource season erodes the stable co-existence equilibrium and results in dominance by one or the other ecotype, depending on which season is removed. We observed similar shifts from co-existence to dominance in three evolutionarily independent populations. In two of these, the stationary phase had no effect on competition between the types, but in the third population, stationary phase favoured one type. Because resources are depleted in each batch, in each season the growth rate of one ecotype depends on the growth rate of the other ecotype. Our results thus strongly support the claim that co-existence between the two ecotypes is maintained by frequency-dependent competition in a seasonal environment.

Keywords: ecological diversification, environmental variability, frequency dependence, seasonality, seasonal resource availability, trade-off.

IF you are connected using the IP of a subscribing institution (library, laboratory, etc.)
or through its VPN.


        © 2007 Christine C. Spencer. All EER articles are copyrighted by their authors. All authors endorse, permit and license Evolutionary Ecology Ltd. to grant its subscribing institutions/libraries the copying privileges specified below without additional consideration or payment to them or to Evolutionary Ecology, Ltd. These endorsements, in writing, are on file in the office of Evolutionary Ecology, Ltd. Consult authors for permission to use any portion of their work in derivative works, compilations or to distribute their work in any commercial manner.

       Subscribing institutions/libraries may grant individuals the privilege of making a single copy of an EER article for non-commercial educational or non-commercial research purposes. Subscribing institutions/libraries may also use articles for non-commercial educational purposes by making any number of copies for course packs or course reserve collections. Subscribing institutions/libraries may also loan single copies of articles to non-commercial libraries for educational purposes.

       All copies of abstracts and articles must preserve their copyright notice without modification.